Articles » Microsatellite analysis of Damask rose (Rosa damascena Mill.) accessions from various regions in Iran reveals multiple genotypes
Previous studies have indicated that all production material in Bulgaria and Turkey consists of only
one genotype. Nine polymorphic microsatellite markers were used to analyze the genetic diversity
of 40 accessions of R. damascena collected across major and minor rose oil production areas in Iran.
Results: All microsatellite markers showed a high level of polymorphism (5–15 alleles per
microsatellite marker, with an average of 9.11 alleles per locus). Cluster analysis of genetic
similarities revealed that these microsatellites identified a total of nine different genotypes. The
genotype from Isfahan province, which is the major production area, was by far the most common
genotype (27/40 accessions). It was identical to the Bulgarian genotype. Other genotypes (each
represented by 1–4 accessions) were collected from minor production areas in several provinces,
notably in the mountainous Northwest of Iran.
Conclusion: This is the first study that uncovered genetic diversity within Damask rose. Our
results will guide new collection activities to establish larger collections and manage the Iranian
Damask rose genetic resources. The genotypes identified here may be directly useful for breeding.